Research Article
Olive Leaves Extract and Oleuropein Improve Insulin Sensitivity in 3T3-L1 Cells and in High-Fat Diet-Treated Rats via PI3K/AkT Signaling Pathway
Table 4
Scoring criteria of immunohistochemistry assay with antibodies including in signaling pathway in liver tissue.
| | p-AKT | | Staining positive cells | Staining intensity | Final score product | | Groups | Percent% | Score 1 | Intensity | Score 2 | Score 1 X Score 2 | Score 3 |
| | CTR | 26%-50% | 2 | Moderate | 2 | 5-8 | 2+(++) | | HFD | 6%-25% | 1 | Weak | 1 | 2-4 | 1+(+) | | HFD + OLA | 26%-50% | 2 | Moderate | 2 | 5-8 | 2+(++) | | p-IRS | | CTR | 51%-75% | 3 | Strong | 3 | 9-12 | 3+(+++) | | HFD | <5% | 0 | Absent | 0 | 0-1 | 0(-) | | HFD + OLA | 51%-75% | 3 | Strong | 3 | 9-12 | 3+(+++) | | GLUT-4 | | CTR | 51%-75% | 3 | Strong | 3 | 9-12 | 3+(+++) | | HFD | <5% | 0 | Absent | 0 | 0-1 | 0(-) | | HFD + OLA | 51%-75% | 3 | Strong | 3 | 9-12 | 3+(+++) |
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Note: scoring results are based on screening 12 consecutive microscopic fields.
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