Improvement of Antioxidant Defences in Keratinocytes Grown in Physioxia: Comparison of 2D and 3D Models

Figure 1

The antioxidant expression profiles are different between HaCaT, NHEK, RHE, and skin explants. HaCaT cells, NHEK, and RHE in normoxia and skin explants were analysed for the expression of mRNA of antioxidant enzymes. The results are expressed as normalized relative mRNA expression () for catalase, superoxide dismutases 1 and 2, and glutathione peroxidases 1 and 4 ( for HaCaT, NHEK, and explants and for RHE). Statistical significance was determined using analysis of variance (ANOVA), comparing each mRNA expression in NHEK to their expression in other models. , , , and . There is no statistical difference for each mRNA expression when comparing RHE and skin explant.