Research Article
Improvement of Antioxidant Defences in Keratinocytes Grown in Physioxia: Comparison of 2D and 3D Models
Figure 2
Differences in HaCaT and NHEK proliferation rates in physioxia vs. normoxia. HaCaT and NHEK were maintained either in physioxia (3% O2) or in normoxia (18.6% O2) for one week. (a) Phase contrast images of HaCaT and NHEK cultures at four days after seeding, scale bar: 400 μm. (b) Proliferation curves of HaCaT and NHEK in physioxia and in normoxia. Cells were quantified using the NucleoCounter® allowing the evaluation of viable cells. Data are represented with of 2 independent experiments including 6 replicates each. Statistical significance was determined using analysis of variance (ANOVA). , , , and .
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