Improvement of Antioxidant Defences in Keratinocytes Grown in Physioxia: Comparison of 2D and 3D Models

Figure 4

Decrease in H₂O₂-induced ROS production in keratinocytes under physioxia condition. HaCaT and NHEK, in physioxia or in normoxia, were submitted to H₂O₂ stress (50 to 400 μM) for 20 min. ROS production was evaluated using the CM-H₂DCFDA probe. Nontreated (NT) cells were incubated with N-acetyl-cysteine (NAC, 5 mM), a ROS scavenger. Basal ROS production was expressed as normalized values versus normoxia condition. H₂O₂-stimulated ROS production was expressed as normalized values versus the corresponding control, normoxia or physioxia. Data are represented with of at least 3 independent experiments. Statistical significance was determined using analysis of variance (ANOVA). ^## and ^#### versus the corresponding control, physioxia or normoxia. , , , and between physioxia and normoxia.