Improvement of Antioxidant Defences in Keratinocytes Grown in Physioxia: Comparison of 2D and 3D Models

Figure 6

Physioxia modulates antioxidant enzyme amounts in keratinocyte cultures. HaCaT and NHEK were submitted to physioxia (3% O₂) or normoxia (18.6% O₂) for 4 days. The catalase, SOD1, SOD2, GPX1, and GPX4 were visualized by red immunofluorescence with a counterstain of nuclei with bisbenzimide in HaCaT (a) and in NHEK (b). Scale bar: 40 μm. Western blotting pictures and quantification of catalase, SOD1, SOD2, and GPX4 in HaCaT (c) and in NHEK (d). Data are represented by the relative protein amount with of at least 3 independent experiments. Statistical significance was determined using analysis of variance (ANOVA). and , between physioxia and normoxia.

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