Curcumin Induces Ferroptosis in Follicular Thyroid Cancer by Upregulating HO-1 Expression

Figure 5

Nthy-ori-3-1 and FTC cells transfected with siHO-1/HO-1-OE and then subjected to curcumin treatment. (a–c) The relative GPX4 expression in Nthy-ori-3-1 and FTC cells transfected with siHO-1/HO-1-OE under 20 μM curcumin treatment. β-Actin was used as an internal control. Relative iron (d–f), GSH (g–i), and MDA (j–l) contents in Nthy-ori-3-1 and FTC cells transfected with siHO-1/HO-1-OE under 20 μM curcumin treatment. (m–o) Determination of lipid ROS levels via fluorescence intensity measurements in Nthy-ori-3-1 and FTC cells transfected with siHO-1/HO-1-OE after treatment with 20 μM curcumin; scale bars: 50 μm. ns: no significant difference compared with the Nc siRNA/vector + curcumin group. and compared with the Nc siRNA + curcumin group. ^# and ^## compared with the vector + curcumin group. The data are presented as the (SEM), . The test was used to determine statistical significance.

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