Research Article
A Multifunctional (-)-Meptazinol-Serotonin Hybrid Ameliorates Oxidative Stress-Associated Apoptotic Neuronal Death and Memory Deficits via Activating the Nrf2/Antioxidant Enzyme Pathway
Figure 4
Effect of Mep-S on the Nrf2/antioxidant enzyme pathway in H2O2-stimulated SH-SY5Y cells. (a) Protein levels of HO-1, NQO-1, Keap1, Akt, phosphorylated Akt, and nuclear and cytoplasmic Nrf2 in SH-SY5Y cells detected by western blot analysis. The cells were pretreated with Mep-S (1 μM) or NAC (1 mM) for 1 h prior to stimulation with H2O2 (400 μM) for 24 h. Data are presented as the of 3 independent experiments. One-way ANOVA: , (HO-1); , (NQO-1); , (p-Akt); , (Keap1); , (N-Nrf2); , (C-Nrf2). and . (b) Interactions (left) and docking representation (right) of Mep-S with the Kelch domain of mouse Keap1 (PDB code: 6ZF4). Mep-S is shown as a gray stick model, and Keap1 is shown as a cartoon. In the 2D diagram (the left panel), key residues of Keap1 are represented as circular shapes and colored according to the interaction types.
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