NF-κB Inhibitor Myrislignan Induces Ferroptosis of Glioblastoma Cells via Regulating Epithelial-Mesenchymal Transformation in a Slug-Dependent Manner

Figure 3

Myrislignan induced the ferroptosis of GBM cells in a system x_c⁻-dependent manner. (a) Cell viability assay was conducted when U87 cells were treated with myrislignan (10 μg/ml), Z-VAD-FMK (20 μM), Necro (20 μM), 3-MA (5 mM), and Fer-1 (10 μM). (b) Cell viability assay was conducted when U87 cells were treated with myrislignan (10 μg/ml), DFO (10 μM), Fer-1 (10 μM), RSL3 (2 μM), and erastin (10 μM). (c–f) The MDA assay, glutathione assay, Cys assay, and liable iron pool assay were conducted to measure the levels of MDA, GSH, Cys, and liable iron in U87 cells treated with increasing concentrations of myrislignan, respectively. (g) U87 cells were cultured in DMED containing increasing concentrations of myrislignan and prepared for transmission electron microscopy observation. (h, i) Protein (SLC7A11, Nrf2, TFR1, and GPX4) levels were detected after treatment with increasing concentrations of myrislignan in U87 cells. ; ; ; ns: no significance.

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