NF-κB Inhibitor Myrislignan Induces Ferroptosis of Glioblastoma Cells via Regulating Epithelial-Mesenchymal Transformation in a Slug-Dependent Manner

Figure 4

Myrislignan regulated the ferroptosis of GBM cells via Slug-SLC7A11 pathway. (a) Protein (SLC7A11, Snail1, and p-p65) levels were detected via western blot assay after myrislignan treatment with transfection of HA-Snail1 in U87 cells. (b, c) Protein (SLC7A11, Slug, and p-p65) levels were detected via western blot assay after myrislignan treatment with transfection of Flag-Slug or sh-Slug in U87 cells. (d–g) The lipid ROS assay, MDA assay, glutathione assay, and Cys assay were conducted to measure the levels of lipid ROS, MDA, GSH, and Cys in U87 cells after myrislignan treatment with transfection of Flag-Slug, respectively. ; ; ; ns: no significance.

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