Photobiomodulation at Defined Wavelengths Regulates Mitochondrial Membrane Potential and Redox Balance in Skin Fibroblasts

Table 1

Irradiation parameters.

Wavelength (nm)	Color	Nominal peak power (mW)	Used LED (data sheets available on request)	Current value for a irradiance of 0.1 mW/cm^2 (mA)
440	UV	19–23 mW	LLS-UV400	3.65
525	Blue	100 cd	G58A5111P	4
645	Red	11 mW	LED-645-03	5.90
660	Red	15 mW	LED-660N-03	10
780	FR/IR	45 mW	ELD-780-525	11
900	IR	48 mW/sr	B5B-900-8	26
The irradiance was adjusted using a ThorLabs PM100D power meter
Main probe characteristics:
Emitter to target distance			120 mm
Spot diameter and area at target distance			15.5–754.4 mm^2
Pulse radiation frequency			50–100–150 Hz
Duty cycle peak irradiance			1% 0.1 mW/cm^2
Mean irradiance			0.001 mW/cm^2
Irradiation time			20 s
Specific energy delivered			0.02 mJ/cm^2

The following table summarizes all the irradiation parameters standardized and controlled by the LED emitter device used in this study.