EGCG Protects against 6-OHDA-Induced Neurotoxicity in a Cell Culture Model

Figure 1

Effect of 6-OHDA and EGCG on N27 cell death, cell viability, and cell apoptosis. Data are represented as % of control (no treatment). Cell death (a) was determined by SYTOX green assay, and values are mean ± SEM, . Cells were pretreated with EGCG for 2 h followed by 100 μM 6-OHDA treatment for 6 h. Bars without common letters differ. Cell viability (b) was evaluated by MTT assay. 6-OHDA, 100 μmoles/L for 6 h; EGCG, 100 μmoles/L. Pre-trt EGCG: pretreatment for 2 h; Co-trt EGCG: cotreatment with 6-OHDA. Values are mean ± SEM, . Cell apoptosis (c) was measured as caspase-3 activity. 6-OHDA (100 μM) for 6 h following EGCG (100 μM) pretreatment for 2 h. Values are mean ± SEM, . Labeled bars without a common letter differ. .

(a)

(b)

(c)