CUB and Sushi Multiple Domains (CSMD1) Gene Polymorphisms and Susceptibilities to Idiopathic Parkinson’s Disease in Northern Chinese Han Population: A Case-Control Study

Table 2

Specific information on the PCR-RFLP reaction process of the three SNPs of CSMD1.

Polymorphisms	Primer sequences (5 ⟶ 3)	PCR conditions (°C/s)			Restriction enzyme digestion	Allele	DNA fragment size (bp)
		Denature	Extension	Annealing
rs12681349	F-TTTGGATGGCAATTATCTACTGC	94/30	60/30	72/60	MseI	C	169
	R-ATAGATAATGCATGCTGGGCT					T	121 + 48
rs10503253	F-AGCAGGTTCAACAGACTTATTTC	94/30	60/30	72/60	MseI	C	221
	R-TCAGAGAAAGCCCTAGTCCC					A	171 + 50
rs1983474	F-GCTATCTGGGCAACCCTTAC	94/30	65/30	72/60	HincII	T	327
	R-TTTCAGCTTCCCTCCACTTAC					G	34 + 293