Cryptochrome and Period Proteins Are Regulated by the CLOCK/BMAL1 Gene: Crosstalk between the PPARs/RXR𝛼-Regulated and CLOCK/BMAL1-Regulated Systems

Figure 4

Dose-dependent changes in activity of PPAR𝛼/RXR𝛼-mediated transcription via the acyl-CoA oxidase (AOX) deletion promoter by the CLOCK/BMAL1 gene (0–90 ng) in human kidney 293T cells. Alterations in PPRE-mediated transcription of AOX by the CLOCK/BMAL1 gene alone (a), plus the PPAR𝛼/RXR𝛼 gene (50 ng) (b), and plus the PPAR𝛼 ligand/activator fenofibric acid (10 𝜇M) (c). The luciferase activity of del-pAOX-Luc was normalized to the luciferase activity of pRL-TK. The del-AOX-Luc plasmid contained no repeat sequences (5^′-AGGTCA-3^′). Experiments were performed in triplicate, and four independent experiments were performed. All data are means ± SD.

(a)

(b)

(c)