Cryptochrome and Period Proteins Are Regulated by the CLOCK/BMAL1 Gene: Crosstalk between the PPARs/RXR𝛼-Regulated and CLOCK/BMAL1-Regulated Systems

Figure 7

The effect of the CLOCK/BMAL1 heterodimer on PPAR𝛾 target genes. Changes in activity of PPAR𝛾1/RXR𝛼-mediated (50 ng) (a) transcription of the cellular retinol binding protein II (CRBPII) promoter by the CLOCK/BMAL1 gene (50 ng) in human kidney 293T cells in the presence and absence of the PPAR𝛾 ligand/activator troglitazone (0–30 𝜇M). The del-0-CRBPII-Luc plasmid contained no repeat sequences (5^′-AG(G/T)TCA-3^′). The luciferase activity of pCRBP-Luc was normalized to the luciferase activity of pRL-TK. Experiments were performed in triplicate, and four independent experiments were performed. All data are means ± SD. ^*P < .05 versus cells not transfected with the CLOCK/BMAL1 gene. Differences were analyzed by repeated-measures ANOVA.

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