Cryptochrome and Period Proteins Are Regulated by the CLOCK/BMAL1 Gene: Crosstalk between the PPARs/RXR-Regulated and CLOCK/BMAL1-Regulated Systems
Figure 8
Inhibition
of CLOCK/BMAL1 by PPAR and PPAR. The PPAR/RXR (a) gene (50 ng) and the PPAR1/RXR (b) gene (50 ng) abolished activation of the Period1 (Per1) promoter by the CLOCK/BMAL1 gene (0–50 ng) in human kidney 293T cells in the presence of the PPAR
ligand/activator fenofibric acid (10 M) (a) and PPAR ligand/activator troglitazone (10 M) (b). The luciferase activity of
pPER-Luc was normalized to the luciferase activity of pRL-TK. Experiments were
performed in triplicate, and four independent experiments were performed. All
data are means ± SD. *P < .05 versus cells not transfected with the PPAR/RXR gene or PPAR/RXR gene. Differences were
analyzed by repeated-measures ANOVA.
