The PPAR-𝜸 Agonist 15-Deoxy-𝚫^12,14-Prostaglandin J₂ Attenuates Microglial Production of IL-12 Family Cytokines: Potential Relevance to Alzheimer's Disease

Figure 3

15d-PGJ₂ inhibits microglial mRNA expression of IL-12p40, IL-12p35, and IL-23p19 induced by A𝛽_1-42 plus LPS. Cells were pretreated for 1 hour with 15d-PGJ₂ (2.5𝜇M). A𝛽_1-42 (10𝜇M), LPS (5 ng/mL), or A𝛽_1-42 (10𝜇M) plus LPS (5 ng/mL) was added as indicated, and 6 hours later, total RNA was isolated. IL-12p35 (a), IL-12p40 (b), and IL-23p19 (c) mRNA levels were determined by real-time quantitative RT-PCR. Results are expressed as fold inductions in GAPDH normalized mRNA values versus levels in LPS-treated cells. Values are mean ± s.e.m of six samples derived from three independent experiments, with each experiment performed in duplicate. ^∗𝑃<.05, ^∗∗𝑃<.01, and ^∗∗∗𝑃<.001 versus A𝛽_1-42 + LPS-treated cultures.

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