The PPAR-𝜸 Agonist 15-Deoxy-𝚫^12,14-Prostaglandin J₂ Attenuates Microglial Production of IL-12 Family Cytokines: Potential Relevance to Alzheimer's Disease

Figure 4

The effects of 15d-PGJ₂ on microglial mRNA expression of CD14, MyD88, TLR4, and TLR2 induced by A𝛽_1-42 plus LPS. Cells were pretreated for 1 hour with 15d-PGJ₂ (2.5𝜇M). A𝛽_1-42 (10𝜇M), LPS (5 ng/mL), or A𝛽_1-42 (10𝜇M) plus LPS (5 ng/mL) was added as indicated, and 6 hours later, total RNA was isolated. CD14 (a), MyD88 (b), TLR4 (c), and TLR2 (d) mRNA levels were determined by real-time quantitative RT-PCR. Results are expressed as fold inductions in GAPDH normalized mRNA values versus levels in LPS-treated cells. Values are mean ± s.e.m of six samples derived from three independent experiments, with each experiment performed in duplicate. ^∗𝑃<.05, ^∗∗𝑃<.01, and ^∗∗∗𝑃<.001 versus A𝛽_1-42 + LPS-treated cultures.

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