Activation of Penile Proadipogenic Peroxisome Proliferator-Activated Receptor with an Estrogen: Interaction with Estrogen Receptor Alpha during Postnatal Development
Table 1
PCR primer sets, sequence, product size (bp), nucleotide (nt) location, and GenBank accession numbers for rat
PPARs used in this study. Note that a common antisense oligoprimer (sequence in bold) was used for PPARγ1a, PPARγ1b, and PPARγ2.
Product/
Sense primer
Antisense primer
Product size
nt
accession#
(bp)
location
PPARα
5-TTG TGA CTG GTC AAG CTC AGG ACA-3
5-TCG TAC GCC AGC TTT AGC CGA ATA-3
492
296–787
NM013196
PPARβ
5-TAA CGC ACC CTT CAT CAT CCA CGA-3
5-TTG ACA GCA AAC TCG AAC TTG GGC-3
390
873–1262
U40064
PPARγ
5-TCT CCA GCA TTT CTG CTC CAC ACT-3
5-ATA CAA ATG CTT TGC CAG GGC TCG-3
533
257–789
NM013124
PPARγ1a
5-CTG ACG AGG TCT CTC TC G GCT G-3
5-AGC AAG GCA CTT CT GAA ACC GA-3
658
21–679
AF246458
PPARγ1b
5-CAG CGC TAA ATT CAT CTT AAC T-3
5-AGC AAG GCA CTT CTG AA A CCG A-3
618
21–639
AF246457
PPARγ2
5-GAG CAT GGT GCC TTC GCT GA-3
5-AGC AAG GCA CTT CTG AA A CCG A-3
563
37–600
AB019561
85–648
AF156666
86–649
Y12882
PPARγ/ERα
(primers for real-time PCR were obtained from Superarray Inc)
