Prevention of Oxidative Stress-Induced Retinal Pigment Epithelial Cell Death by the PPAR𝛾 Agonists, 15-Deoxy-Delta 12, 14-Prostaglandin J₂

Figure 1

Prevention of H₂O₂-induced nuclear condensation by 15d-PGJ₂. The human RPE cell line ARPE-19 cells were treated with 1.5 mM H₂O₂ for various periods of time, and then processed for nuclear staining by bisbenzimide (Hoechst 33258) to identify apoptotic cells [48]; (a): untreated cells; (b): 4 hours; (c): 12 hours; (d): 16 hours after treatment. Arrows in (c) point to representative cells with condensed nuclei, an indication of apoptosis. (e): Cells were pretreated with 1 𝜇M 15d-PGJ₂ overnight, followed by 1.5 mM H₂O₂ for 16 hours (without 15d-PGJ₂). The number of apoptotic cells was greatly reduced by 15d-PGJ₂. Scale bar: 100 𝜇m.

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