A Dominant-Negative PPAR Mutant Promotes Cell Cycle Progression and Cell Growth in Vascular Smooth Muscle Cells
Figure 4
Ad-DN up-regulates, while Ad-WT and Ad-CA down-regulate, positive regulators of G0/G1, S, and G2/M phases. CASMCs were arrested in serum-deprived medium for 24 hours, then infected with or without Ad-DN, -WT, -GFP, -VP16, or -CA recombinant adenoviruses at the indicated MOIs for 48 hours, with or without serum stimulation. Western blot analyses were performed on whole cell extracts of these samples using antibodies against the indicated cell cycle regulatory proteins, then stripped and rehybridized with a -actin specific antibody to assess loading variability. Western blots shown are representative of at least four independent experiments.