The PPAR Ligand GW501516 Reduces Growth but Not Apoptosis in Mouse Inner Medullary Collecting Duct Cells
Figure 7
GW501516 does not significantly alter IMCD-K2 cell viability. Serum-starved cells were stimulated
for 24 hours with serum-free media (control) or increasing concentrations of
GW501516 ( to M). The cells were then incubated
for one hour with the DNA dye-binding solution from the CyQUANT NF assay kit
(Invitrogen). Fluorescence intensity, representing number of cells, was then
measured as per the manufacturer’s instructions. Values (fold control) are means S.E.M.;
.
