Activation of PPARs , /, and Impairs TGF-1-Induced Collagens' Production and Modulates the TIMP-1/MMPs Balance in Three-Dimensional Cultured Chondrocytes
Figure 4
Effects of synthetic PPAR agonists on TIMP-1 production and changes of global MMPs (-1, -2, -3, -7, and -9) and specific MMP-13 activities induced by in rat chondrocytes. Chondrocytes, embedded in alginate beads (5.104 cells per bead), were exposed to Wy14643 (100 M), GW501516 (100 nM), or rosiglitazone (10M) for 2 h before stimulation with TGF-1 (10 ng/mL), in the presence or absence of PPAR agonists, for 72 h. (a) Global activity of MMPs (-1, -2, -3, -7, and -9) in culture supernatants by fluorimetric assay (); (b) Activity of MMP-13 in culture supernatants by specific fluorimetric assay (); (c) TIMP-1 protein levels in culture supernatants by ELISA (). Data were expressed as mean ± SEM and statistically significant differences from the control were indicated as and from TGF-1-treated cells as .
