Changes in the 24 h Rhythmicity of Liver PPARs and Peroxisomal
Markers When Feeding Is Restricted to Two Daytime Hours
Figure 2
Effect of restricted feeding on daily expression of liver PPARs. Double-plotted representation of diurnal expression in liver homogenates (50 μg of protein) of (a) PPARα, (b) PPARβ, and (c) PPARγ. Control group fed ad libitum (AL, ●) and group with RF (∘). Results were normalized to the content of β-actin in parallel determinations to adjust for loading differences. Representative western blot experiments are displayed at the right of each graph. Data are presented as mean ± SEM of 4 rats from each time point. Black bar depicts the dark period (from 20:00 to 08:00 h), and the gray bar indicates the time of food access (from 12:00 to 14:00 h). Time points with a significant difference between ad libitum and RF groups are marked with an asterisk (*) (Tukey post hoc test, ).
