Changes in the 24 h Rhythmicity of Liver PPARs and Peroxisomal
Markers When Feeding Is Restricted to Two Daytime Hours
Figure 3
Effect of fasting and refeeding on liver PPARs. Liver homogenates (50 μg of protein) were analyzed for the expression of PPARα, PPARβ, and PPARγ. Results were normalized to the content of β-actin in parallel determinations to adjust for loading differences. Fasted groups: RF at 11:00 h, 21 h and 45 h without food, and refeeding groups: RF at 14:00 h, 21 h + 2 h of food access, and 45 h + 2 h of food access. Data are presented as mean ± SEM of 4 rats from each temporal point. Each group of fasted and refed groups was compared by one-way ANOVA (Tukey post hoc test, ); significant differences between fasting versus refed groups were detected by the Student’s -test- ().
