Changes in the 24 h Rhythmicity of Liver PPARs and Peroxisomal
Markers When Feeding Is Restricted to Two Daytime Hours
Figure 4
Effect of restricted feeding on daily expression of liver peroxisomal markers. Double-plotted representation of diurnal expression in liver homogenates (50 μg of protein) of (a) PMP70, (b) AOX, and (c) catalase activity. Control group fed ad libitum (AL, ●) and group with RF (∘). Results for PMP70 and AOX were normalized to the content of β-actin determined in parallel determinations to adjust for loading differences. Representative western blots for PMP 70 and AOX are displayed on the right. Data are presented as mean ± SEM of 4 rats from each time point. Black bar depicts the dark period (from 20:00 to 08:00 h), and the gray bar indicates the time of food access (from 12:00 to 14:00 h). Time points with significant difference between ad libitum and RF groups are depicted with an asterisk (*) (Tukey post hoc test, ).
