Changes in the 24 h Rhythmicity of Liver PPARs and Peroxisomal
Markers When Feeding Is Restricted to Two Daytime Hours
Figure 5
Effect of fasting and refeeding on liver peroxisomal markers. Liver homogenates (50 μg of protein) were analyzed for the expression of PMP70, AOX, and catalase activity. Results for PMP70 and AOX were normalized to the content of β-actin in parallel determinations to adjust for loading differences (or as above). Fasted groups: RF at 11:00 h, 21 h, and 45 h without food, and refeeding groups: RF at 14:00 h, 21 h + 2 h of food access, and 45 h + 2 h of food access. Data are presented as mean ± SEM of 4 rats from each time point. The fasted and refed groups were compared by one-way ANOVA (Tukey post hoc test, ); significant differences for fasting versus refed groups were detected by the Student’s -test ().
