AS601245, an Anti-Inflammatory JNK Inhibitor, and Clofibrate Have a Synergistic Effect in Inducing Cell Responses and in Affecting the Gene Expression Profile in CaCo-2 Colon Cancer Cells

Table 1

Top ten genes^b.

(a) Clofibrate

Gene ID	Gene name	Fold change
GANAB*	Glucosidase, alpha; neutral AB	+3.8
ATF6B	Activating transcription factor 6 beta	+2.8
SETD5	SET domain containing 5	+2.6
NFAT5	Nuclear factor of activated T cells 5, tonicity-responsive	+2.5
DLST	Dihydrolipoamide S-succinyltransferase (E2 component of 2-oxoglutarate complex)	+2.3
IL6ST	Interleukin 6 signal transducer (gp130, oncostatin M receptor)	+2.1
HNRNPA0	Heterogeneous nuclear ribonucleoprotein A0	+2.0
MEX3D	Mex-3 homolog D (C. elegans)	+1.6
CTSZ	Cathepsin Z	+1.5
STIP 1	Stress-induced phosphoprotein 1	−3.1
CLASP 1	Cytoplasmic linker-associated protein 1	−3.1
MACF 1	Microtubule-actin cross-linking factor 1	−2.9
COPA	Coatomer protein complex, subunit alpha	−2.7
HNRNPA1	Heterogeneous nuclear ribonucleoprotein A1	−2.6
SPSB2	SplA/ryanodine receptor domain and SOCS box containing 2	−2.3
TSR1	TSR1, 20S rRNA accumulation, homolog (S. cerevisiae)	−2.3
VIL1*	Villin 1	−2.3
CDH1	Cadherin 1, type 1, E-cadherin (epithelial)	−2.2
CRTAP	Cartilage-associated protein	−2.2

(b) AS601245

Gene ID	Gene name	Fold change
CYP1A1	Cytochrome P450, family 1, subfamily A, polypeptide 1	+5.3
AP3D1	Adaptor-related protein complex 3, delta 1 subunit	+3.6
NFAT5	Nuclear factor of activated T cells 5, tonicity-responsive	+3.1
BMP2K	Bone morphogenetic protein 2 inducible kinase	+2.9
DLST	Dihydrolipoamide S-succinyltransferase (E2 component of 2-oxoglutarate complex)	+2.8
IL6ST	Interleukin 6 signal transducer (gp130, oncostatin M receptor)	+2.8
FAM76B	Family with sequence similarity 76, member B	+2.4
MGA	MAX gene associated	+2.4
THRAP3	Thyroid hormone receptor-associated protein 3	+2.4
GANAB	Glucosidase, alpha; neutral AB	+2.3
RPS27A	Ribosomal protein S27a	−4.2
HNRNPA1	Heterogeneous nuclear ribonucleoprotein A1	−4.1
WDR33	WD repeat domain 33	−3.9
EPN1	Epsin 1	−3.7
KLC2	Kinesin light chain 2	−3.6
STIP1	Stress-induced phosphoprotein 1	−3.6
SEPT9	Septin 9	−3.4
TFDP1	Transcription factor Dp-1	−3.1
LAD1	Ladinin 1	−3.1
CABIN 1	Calcineurin-binding protein 1	−3.1

(c) Clofibrate + AS601245

Gene ID	Gene name	Fold change
CYP1A1	Cytochrome P450, family 1, subfamily A, polypeptide 1	+7.5
IL6ST	Interleukin 6 signal transducer (gp130, oncostatin M receptor)	+6.4
AP3D1	Adaptor-related protein complex 3, delta 1 subunit	+5.1
WASF2	WAS protein family, member 2	+4.1
VAPB	VAMP- (vesicle-associated membrane-protein-)associated protein B and C	+3.9
NFAT5*	Nuclear factor of activated T cells 5, tonicity-responsive	+3.8
THRA	Thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian)	+3.6
GANAB*	Glucosidase, alpha; neutral AB	+3.6
BDP1	B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB	+3.4
SMARCC1	SWI/SNF related, matrix associated, actin-dependent regulator of chromatin, subfamily c, member 1	+3.2
TRUB1	TruB pseudouridine (psi) synthase homolog 1 (E. coli)	−4.4
HNRNPA1	Heterogeneous nuclear ribonucleoprotein A1	−3.9
SLC39A14	Solute carrier family 39 (zinc transporter), member 14	−3.6
NXT2	Nuclear transport factor-2-like export factor 2	−3.4
MALAT1	Metastasis-associated lung adenocarcinoma transcript 1 (nonprotein coding)	−3.2
LARP5	La ribonucleoprotein domain family, member 5	−3.0
FGB	Fibrinogen beta chain	−2.8
ACTC1	Actin, alpha, cardiac muscle 1	−2.8
TFRC	Transferrin receptor (p90, CD71)	−2.8
RPS27A	Ribosomal protein S27a	−2.7

^bThe top ten genes upregulated and downregulated the most by 5 μM Clofibrate (Table 1(a)), 0.1 μM AS601245 (Table 1(b)), and combined treatment (Table 1(c)) with respect to DMSO-treated Caco-2 cells at 24 h.