|
| Gene description | Primers | Sequences (5′→3) | Annealing
temperature(°C) | PCR
product (bp) |
|
| Peroxisome proliferator-activated receptor gamma2 (PPARγ2) | F
R | TTCGGAATCAGCTCTGTGGA
CCATTGGGTCAGCTCTTGTG | 55 | 148 |
| CCAAT/enhancer-binding protein, alpha (C/EBPα) | F
R | AAGGCCAAGAAGTCGGTGGA
CCATAGTGGAAGCCTGATGC | 55 | 189 |
| Adipocyte protein 2 (aP2) | F
R | ACATGAAAGTGGGAGTG
AAGTACTCTCTGACCGGATG | 55 | 128 |
| Leptin | F
R | CTCCAAGGTTGTCCAGGGTT
AAAACTCCCCACAGAATGGG | 55 | 143 |
| Sterol regulatory element-binding factor 1 (SREBP1c) | F
R | TTGTGGAGCTCAAAGACCTG
TGCAAGAAGCGGATGTAGTC | 55 | 94 |
| Lipoprotein lipase (LPL) | F
R | TGCCGCTTTTTGTTTTACC
TCACAGTTTCTGCTCCCAGC | 55 | 172 |
| Secreted frizzled-related protein 5 (SFRP5) | F
R | CTTGGTGTCCTTGCGCTTTA
CTGATGGCCTCATGGAACAG | 61 | 155 |
| Wingless-type MMTV integration site family, member 10B (WNT10b) | F
R | TTTTGGCCACTCCTCTTCCT
TCCTTTTCCAACCGAAAACC | 61 | 183 |
| Dickkopf 2 (DKK2) | F
R | GCATTTCCTTCAGATTGGCA
TCATTCCCTGTTCTTCAGCG | 55 | 144 |
| Galanin receptor 1 (GalR1) | F
R | CCAAGGGGGTATCCCAGTAA
GGCCAAACA CTACCAGCGTA | 55 | 147 |
| Galanin receptor 2 (GalR2) | F
R | ATAGTGGTGCTCATGCTGGAA
AGGCTGGATCGAGGGTTCTA | 60 | 134 |
| Protein kinase C δ (PKCδ) | F
R | CTGAGCGCTGCAAGAAGAAC
TGGAAACTTTGATCCTGCACTGA | 60 | 146 |
| Cyclin D (Cyc-D) | F
R | TGGGAAGTTTTGTTGGGTCA
TCCTTGTCCAGGTAATGCCA | 55 | 144 |
| E2F1 | F
R | CCTGGAGCATGTTAAAGAAG
CCTCGAGACCAAAGTGATAG | 48.9 | 102 |
| Toll-like receptor 2 (TLR2) | F
R | TCTAAAGTCGATCCGCGACA
TACCCAGCTCGCTCACTACG | 56.2 | 344 |
| Toll-like receptor 4 (TLR4) | F
R | ACCTCTGCCTTCACTACAGA
AGGGACTTCTCAACCTTCTC | 48.6 | 223 |
| Myeloid differentiation primary response gene 88 (MyD88) | F
R | AAGAAAGTGAGTCTCCCCTC
TCCCATGAAACCTCTAACAC | 55 | 149 |
| Toll-interleukin 1 receptor domain-containing adaptor protein (Tirap) | F
R | GTGGCCGCTGGAGCAAAGAC
TTGCCTCTGCCATCCACATA | 55 | 370 |
| TNF receptor-associated factor 6 (TRAF6) | F
R | GCACAAGTGCCCAGTTGACA
AGTGTCGTGCCAAGTGATTC | 62.7 | 479 |
| TIR-domain-containing adapter-inducing interferon-β (TRIF) | F
R | ATGGATAACCCAGGGCCTT
TTCTGGTCACTGCAGGGGAT | 56.5 | 528 |
| Interferon regulatory factor 5 (IFR5) | F
R | AATACCCCACCACCTTTTGA
TTGAGATCCGGGTTTGAGAT | 53.1 | 191 |
| Interferon α (IFNα) | F
R | ATGGCTAG(G/A)CTCTGTGCTTTCCT
GGGCTCTCCAGA(T/C)TTCTGCTCTG | 60.2 | 500 |
| Tumor necrosis factor alpha (TNFα) | F
R | TGTCTCAGCCTCTTCTCATT
AGATGATCTGAGTGTGAGGG | 55 | 156 |
| Intereukin 6 (IL-6) | F
R | TTGCCTTCTTGGGACTGATG
CCACGATTTCCCAGAGAACA | 55 | 162 |
| Forkhead box transcription factor O1 (FOXO1) | F
R | TCCCAATGGCACAGTCCTTA
AGCAGTCCAAAGATGCCCTT | 55.2 | 185 |
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | F
R | AGAACATCATCCCTGCATCC
TCCACCACCCTGTTGCTGTA | 60 | 321 |
|
