Research Article
The Nitrated Fatty Acid 10-Nitro-oleate Diminishes Severity of LPS-Induced Acute Lung Injury in Mice
Table 1
Oligonucleotide primers employed.
| | Gene | | Primer sequence | Tm °C | Amplicon size (bp) |
| | 9s rRNA | F | 5′-ATCCGCCAACGTCACATT-3′ | 57.6 | 115 | | R | 5′-CCGCCGCCATAAGGAGAAC-3′ | 64.5 | | CD36 | F | 5′-CAGTCGGAGACATGCTTATTGAG-3′ | 60.7 | 151 | | R | 5′-TTTGCCACGTCATCTGGGTTT-3′ | 62.5 | | COX-2 | F | 5′-TGTGACTGTACCCGGACTGG-3′ | 63.0 | 233 | | R | 5'-TGCACATTGTAAGTAGGTGGAC-3′ | 60.0 | | FABP4 | F | 5′-GGGGCCAGGCTTCTATTCC-3′ | 61.8 | 114 | | R | 5′-GGAGCTGGGTTAGGTATGGG-3′ | 61.1 | | GAPDH | F | 5′-GGACGCATTGGTCGTCTGG-3′ | 63.0 | 204 | | R | 5′-TTTGCACTGGTACGTGTTGAT-3′ | 60.2 | | IL-12 p40 | F | 5′-CAAGGCTGTTCACATTATCCCA-3′ | 60.3 | 107 | | R | 5′-CCAGTGTGGTCATGGACTTTC-3′ | 60.6 | | MCP-1 | F | 5′-TTAAAAACCTGGATCGGAACCAA-3′ | 60.1 | 121 | | R | 5′-GCATTAGCTTCAGATTTACGGGT-3′ | 60.7 | | NOS2 | F | 5′-ACATCGACCCGTCCACAGTAT-3′ | 62.7 | 177 | | R | 5′-CAGAGGGGTAGGCTTGTCTC-3′ | 61.0 | | NOX4 | F | 5′-TGCCTGCTCATTTGGCTGT-3′ | 62.2 | 180 | | R | 5′-CCGGCACATAGGTAAAAGGATG-3′ | 60.8 | | PPAR- | F | 5′-CCATTCTGGCCCACCAAC-3′ | 66.5 | 479 | | R | 5′-CTGAAACCGACAGTACTG-3′ | 53.8 | | TNF- | F | 5′-CCTGTAGCCCACGTCGTAG-3′ | 61.5 | 148 | | R | 5′-GGGAGTAGACAAGGTACAACCC-3′ | 61.4 |
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