Role of Peroxisome Proliferator-Activated Receptor and B-Cell Lymphoma-6 in Regulation of Genes Involved in Metastasis and Migration in Pancreatic Cancer Cells
Figure 4
GW501516 treatment reduces -stimulated Miapaca-2 and BxPc-3 cell invasion through a basement membrane. Human pancreatic cancer cells were transiently infected with the indicated shRNAs and stimulated with human with or without GW501516. Cells were allowed to invade a basement membrane overnight, and relative invasion was quantified using the CytoSelect 96-well cell invasion assay with fluorometric readings at 480 nm520 nm. GW501516 treatment inhibits the invasion of the COX-2 negative pancreatic cancer cell line Miapaca-2 (a) and the COX-2 positive pancreatic cancer cell line BxPc-3 (b) through a basement membrane.
