Identification of Bexarotene as a PPAR Antagonist with HDX

Figure 2

Differential HDX of PPARγ with Bexarotene: (a) residues colored corresponding to the average percent change in deuteration between apo and Bexarotene bound PPARγ over 6 time points (10, 30, 60, 300, 900, and 3600 seconds) run in triplicate () overlaid on PDB:1K74. HDX buildup curves of (b) PPARγ LBD helix 3 peptide IRIFQGCQ (blue) and (c) PPARγ LBD helix 11 RXIVTEHVQL (orange).

(a)

(b)

(c)