Identification of Bexarotene as a PPAR<svg  xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg"   style="vertical-align:-4.26851pt" id="M1" height="12.2747pt" version="1.1" viewBox="-0.0657574 -8.00619 9.07093 12.2747" width="9.07093pt"><g transform="matrix(.017,0,0,-0.017,0,0)"><path id="g5-225" d="M498 369C498 423 469 454 440 454C415 454 388 432 388 408C388 398 391 390 395 384C401 375 413 354 413 330C413 269 332 132 280 70H278C285 133 286 231 273 319C259 412 231 454 191 454C147 454 85 416 21 322L49 293C91 339 110 351 119 351C129 351 132 349 142 324C181 225 194 56 178 -19C148 -80 115 -167 107 -224L125 -246C167 -243 241 -219 265 -202C260 -169 255 -95 256 -21C261 -11 268 2 323 70C438 212 498 291 498 369Z" /><glyph.data ascent="3481" descent="-2878" horiz-adv-x="519" vert-adv-y="519" /></g></svg> Antagonist with HDX

<div>Biochemical characterization of Bexarotene on PPAR<i>γ</i>: (a) TR-FRET competitive displacement assay (<svg height="8.55521pt" id="M10" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z" id="g113-111"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="502" vert-adv-y="502"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z" id="g117-34"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="587" vert-adv-y="587"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M285 378C315 398 338 416 353 432C373 451 384 474 384 503C384 579 325 635 236 635H235C182 635 136 610 108 579L65 516L85 496C110 533 150 575 205 575C258 575 300 543 300 481C300 407 232 369 141 339L147 310C163 315 188 321 211 321C268 321 338 284 338 192C338 94 288 40 217 40C160 40 119 68 93 91C85 98 77 97 69 91C60 84 47 71 46 58C44 46 48 35 62 22C75 10 116 -12 162 -12C234 -12 424 62 424 224C424 297 373 359 285 376V378Z" id="g113-52"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="480" vert-adv-y="480"></glyph.data></g></svg>). (b) Dose dependent transcriptional activity of a PPAR<i>γ</i>:PPRE-luciferase promoter-reporter assay in HEK293T cells (<svg height="8.55521pt" id="M11" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z" id="g113-111"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="502" vert-adv-y="502"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z" id="g117-34"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="587" vert-adv-y="587"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M456 178V225H360V632H320C217 496 115 347 20 206V178H280V106C280 40 276 34 189 27V0H445V27C364 34 360 39 360 106V178H456ZM280 225H82C149 335 214 431 278 520H280V225Z" id="g113-53"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="480" vert-adv-y="480"></glyph.data></g></svg>). (c) Dose dependent transcriptional activity of rosiglitazone ±1 <i>μ</i>M Bexarotene in a PPAR<i>γ</i>:PPRE-luciferase promoter-reporter assay in HEK293T cells (<svg height="8.55521pt" id="M12" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z" id="g113-111"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="502" vert-adv-y="502"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z" id="g117-34"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="587" vert-adv-y="587"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M456 178V225H360V632H320C217 496 115 347 20 206V178H280V106C280 40 276 34 189 27V0H445V27C364 34 360 39 360 106V178H456ZM280 225H82C149 335 214 431 278 520H280V225Z" id="g113-53"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="480" vert-adv-y="480"></glyph.data></g></svg>).</div>

PPAR Research

fig3

Figure 3

Figure 3: Identification of Bexarotene as a PPAR<svg  xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg"   style="vertical-align:-4.26851pt" id="M1" height="12.2747pt" version="1.1" viewBox="-0.0657574 -8.00619 9.07093 12.2747" width="9.07093pt"><g transform="matrix(.017,0,0,-0.017,0,0)"><path id="g5-225" d="M498 369C498 423 469 454 440 454C415 454 388 432 388 408C388 398 391 390 395 384C401 375 413 354 413 330C413 269 332 132 280 70H278C285 133 286 231 273 319C259 412 231 454 191 454C147 454 85 416 21 322L49 293C91 339 110 351 119 351C129 351 132 349 142 324C181 225 194 56 178 -19C148 -80 115 -167 107 -224L125 -246C167 -243 241 -219 265 -202C260 -169 255 -95 256 -21C261 -11 268 2 323 70C438 212 498 291 498 369Z" /><glyph.data ascent="3481" descent="-2878" horiz-adv-x="519" vert-adv-y="519" /></g></svg> Antagonist with HDX 

PPAR Research

Identification of Bexarotene as a PPAR Antagonist with HDX

Figure 3