Timcodar (VX-853) Is a Non-FKBP12 Binding Macrolide Derivative That Inhibits PPARγ and Suppresses Adipogenesis

Figure 2

Rapamycin and timcodar reduce lipid accumulation in 3T3-L1 adipocytes. (a) Cell growth of 3T3-L1 cells in regular BCS growth serum for 48 hours with vehicle (Ctrl), 0.1 and 1.0 μM FK506, 0.1 and 1.0 μM timcodar, and 0.1 and 1.0 μM rapamycin (ANOVA, ). Growth was measured as MTT. ; ; (versus control); ; and (timcodar versus rapamycin treatment) (±S.E.; ). (b) Nile Red staining of lipid accumulation in differentiated 3T3-L1 adipocytes treated with vehicle (0 μM) and increasing doses of timcodar (VX) (0.1 μM or 1.0 μM); (versus control) (±S.E.; ). (c) Comparison of lipid accumulation of differentiated 3T3-L1 adipocytes treated with vehicle (Ctrl), 1 μM FK506 (FK), 1 μM timcodar (VX), and 1 μM rapamycin (Rap); (versus control) (±S.E.; ).

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