Timcodar (VX-853) Is a Non-FKBP12 Binding Macrolide Derivative That Inhibits PPARγ and Suppresses Adipogenesis

Figure 4

Timcodar and rapamycin reduce expression of adipogenic regulators. Western blot and densitometry for (a) PPARγ and (b) C/EBPα, as well as real-time PCR analysis of differentiated 3T3-L1 adipocytes treated with vehicle (Ctrl), 1 μM FK506 (FK), 1 μM timcodar (VX), and 1 μM rapamycin (Rap): (a) PPARγ, (b) C/EBPα, (c) PREF-1, and (d) C/EBPβ. Real-time PCR for a dose-dependent increase of timcodar for treatments of 0 μM, 0.1 μM, or 1.0 μM in gray boxes: (a) PPARγ, (b) C/EBPα, (c) PREF-1, and (d) C/EBPβ. ; ; ; (versus control); ; and (timcodar versus rapamycin treatment) (±S.E.; ).

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