Timcodar (VX-853) Is a Non-FKBP12 Binding Macrolide Derivative That Inhibits PPARγ and Suppresses Adipogenesis

Figure 5

Genes involved in lipid accumulation are suppressed by timcodar. Real-time PCR analysis of differentiated 3T3-L1 adipocytes treated with vehicle (Ctrl), 1 μM FK506 (FK), 1 μM timcodar (VX), and 1 μM rapamycin (Rap), as well as a dose-dependent increase of timcodar for treatments of 0 μM, 0.1 μM, or 1.0 μM in gray boxes. (a) FASN, (b) SREBP1, (c) SCD1, and (d) FABP4. ; ; (versus control); ; and (timcodar versus rapamycin treatment) (±S.E.; ).

(a)

(b)

(c)

(d)