Puerarin Protects against Cardiac Fibrosis Associated with the Inhibition of TGF-β1/Smad2-Mediated Endothelial-to-Mesenchymal Transition

Figure 1

Pressure overload-induced cardiac fibrosis was alleviated in puerarin-treated mice. (a) Histological sections of the left ventricle in indicated groups were stained with PSR for fibrosis (upper and middle panel, scale bars: 100 μm). α-SMA was detected with immunohistochemistry (lower panel, scale bars: 200 μm). (b) TGF-β1/Smad2 signaling pathway and α-SMA protein in indicated groups were determined by WB, normalized to GAPDH . versus sham + vehicle group; versus TAC + vehicle group.

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