Puerarin Protects against Cardiac Fibrosis Associated with the Inhibition of TGF-β1/Smad2-Mediated Endothelial-to-Mesenchymal Transition

Figure 6

PPAR-γ protein expression was upregulated by puerarin. (a) PPAR-γ protein levels in mice hearts in indicated groups were detected by WB, normalized to GAPDH . versus sham + vehicle group; versus TAC + vehicle group. (b) HUVECs were preincubated with different concentrations of puerarin (10, 25, 50 μM) for 30 min and then treated with TGF-β1 (10 ng/ml) for 48 h. PPAR-γ protein levels in cell lysates in indicated groups were detected by WB, normalized to GAPDH . versus control group; versus TGF-β1 group.

(a)

(b)