Quercetin and Quercetin-Rich Red Onion Extract Alter Pgc-1α Promoter Methylation and Splice Variant Expression

Figure 4

Bisulfite sequencing of the Pgc-1α promoter was used to determine DNA methylation levels at the −260 nt. The original (top line) and bisulfite converted (corresponding bottom line) sequences are shown in (a). The bold and underlined region within the bisulfite converted sequence indicates where the pyrosequencing primer is located. The red highlighted, bold, and underlined region is the regulatory −260 nt that undergoes DNA methylation. The percent of DNA methylation present at the −260 nt in skeletal muscle is shown as the mean ± SEM for low fat (LF), high fat (HF), high fat plus quercetin (HF + Q), and high fat plus red onion extract (HF + RO) animals after 9 wks of feeding the respective diets. denotes significant difference with P < 0.05 and P < 0.01.

(a)

(b)