Caffeoylquinic Acid-Rich Extract of Aster glehni F. Schmidt Ameliorates Nonalcoholic Fatty Liver through the Regulation of PPARδ and Adiponectin in ApoE KO Mice

Figure 3

The mRNA levels of peroxisome proliferator-activated receptor delta (PPARδ), 5′ adenosine monophosphate-activated protein kinase α1 (AMPKα1), interleukin 6 (IL6), and tumor necrosis factor α (TNFα) in liver of ApoE KO mice treated with 0.15% cholesterol diet and Aster glehni extract. Total RNA was extracted by Trisol reagent from liver tissues of ApoE KO mice, and complementary DNA was synthesized by cDNA synthesis kit from the total RNA. Polymerase chain reaction (PCR) was done with cDNA, primers, and PCR Premix solution. The PCR image density was analyzed with Image J program. The results are expressed as means ± SEM. Values were statistically analyzed by unpaired -test and one way ANOVA. All experiments were repeated three times. versus control, versus control, and versus control.

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