Caffeoylquinic Acid-Rich Extract of Aster glehni F. Schmidt Ameliorates Nonalcoholic Fatty Liver through the Regulation of PPARδ and Adiponectin in ApoE KO Mice

Figure 4

The protein levels of peroxisome proliferator-activated receptor delta (PPARδ), 5′ adenosine monophosphate-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC), ATP citrate lyase (ACLY), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), and fatty acid synthase (FASN) in liver of ApoE KO mice treated with 0.15% cholesterol diet and Aster glehni extract. Protein extracts were electrophoresed in 10% polyacrylamide gel and blotted to nitrocellulose membrane. The nitrocellulose membrane was bound with primary and secondary antibodies sequentially, and then the chemiluminescence was exposed to X-ray film. The density for bands on X-ray film was analyzed with Image J program. The results are expressed as means ± SEM. Values were statistically analyzed by unpaired -test and one way ANOVA. All experiments were repeated three times. versus control, versus control, and versus control.

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