Caffeoylquinic Acid-Rich Extract of Aster glehni F. Schmidt Ameliorates Nonalcoholic Fatty Liver through the Regulation of PPARδ and Adiponectin in ApoE KO Mice

Figure 5

Immunohistochemistry for TNFα, SOD, HMGCR, and 4-HNE on frozen sectioned slides of liver in ApoE KO mice treated with 0.15% cholesterol diet and Aster glehni extract. (a) Liver tissue slides of ApoE KO mice were fixed and immunohistochemically stained with antibodies of TNFα, SOD, HMGCR, and 4-HNE. Images were taken at 200x magnification. (b) Densities for images were analyzed with Image J program. The results are expressed as means ± SEM. Values were statistically analyzed by unpaired -test and one way ANOVA. All experiments were repeated three times. versus control and versus control.

(a)

(b) Image density