Caffeoylquinic Acid-Rich Extract of Aster glehni F. Schmidt Ameliorates Nonalcoholic Fatty Liver through the Regulation of PPARδ and Adiponectin in ApoE KO Mice

Figure 7

Oil Red O staining result and the protein levels of PPARδ, AMPK and PGC-1α in HepG2 cells treated with 0.2 mM cholesterol, 0.1 mM palmitate, 50 ug Aster glehni extract, and 50 uM GSK0660 (PPARδ antagonist). The lipids in HepG2 (a) were stained with Oil Red O reagent and observed by optical microscope. Accumulated lipid contents in HepG2 cells (b) were eluted by isopropanol and the absorbance of eluted Oil Red O was estimated with ELISA reader at the wave length of 530 nm. Magnification is 200 times. Meaning of indications: Ctrl is an untreated control group, V-Ctrl is a vehicle control group, Cho + Pal is a cholesterol and palmitate treated group, AG is a cholesterol, palmitate, and A. glehni treated group, and GSK is a cholesterol, palmitate, A. glehni, and GSK0660 treated group. Protein extracts were electrophoresed in 10% polyacrylamide gel and blotted to nitrocellulose membrane. The nitrocellulose membrane was bound with primary and secondary antibodies sequentially, and then the chemiluminescence was exposed to X-ray film. The density for bands on X-ray film was analyzed with Image J program (c, d, e). The results are expressed as means ± SEM. Values were statistically analyzed by unpaired -test and one way ANOVA. All experiments were repeated three times. , , and .

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