Caffeoylquinic Acid-Rich Extract of Aster glehni F. Schmidt Ameliorates Nonalcoholic Fatty Liver through the Regulation of PPARδ and Adiponectin in ApoE KO Mice

Figure 8

The protein levels of PPARγ and PPARα in HepG2 cells treated with 0.2 mM cholesterol, 0.1 mM palmitate, and 50 ug Aster glehni extract, and PPARδ and AMPK protein levels in HepG2 cells treated with 10 uM compound C (AMPK antagonist). (a) Liver tissue slides of ApoE KO mice were fixed and immunohistochemically stained with antibody of PPARγ. Images were taken at 200x magnification. (b, c) Protein extracts were electrophoresed in 10% polyacrylamide gel and blotted to nitrocellulose membrane. The nitrocellulose membrane was bound with primary and secondary antibodies sequentially, and then the chemiluminescence was exposed to X-ray film. The density for bands on X-ray film was analyzed with Image J program. The results are expressed as means ± SEM. Values were statistically analyzed by unpaired -test and one way ANOVA. All experiments were repeated three times. and .

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