New Transcriptional Reporters to Quantify and Monitor PPARγ Activity

Table 1

Primers used to amplify DNA fragments by PCR for vector construction, confirmation, and generation of cloned fragments.

Primer name	Sequence
PPRE-AseI	ATTAATAATGCCTGCAGGTCAATTCTG
PPRE-NheI	GCTAGCGATCGCAGATCCTCTAGAGTCC
PPRE-KpnI	CGGGGTACCATGCCTGCAGGTCAATTCTG
PPRE-BglII	GAAGATCTGATCGCAGATCCTCTAGAGTCC
pNL_F	AGGCTGTCCCCAGTGCAAGT
pNL_R	CGGATTGCCAAGCTTGGC
H2B_F	CACCATGCCAGAGCCAG
H2B_R	CTTAGCGCTGGTGTACTTG
GFP_F	CATGGTCCTGCTGGAGTTCGTG
GFP_R	CGTCGCCGTCCAGCTCGACCAG

Restriction enzyme sites added to primer 5′-ends are underlined.