Fluid Retention Caused by Rosiglitazone Is Related to Increases in AQP2 and αENaC Membrane Expression

Figure 2

Altered localization of PPAR in response to rosiglitazone. (a, c) Control in HEK293 and mIMCD-3 cells, respectively. PPAR immunoreactivity following 24 h of culture without rosiglitazone; (b, d) PPAR immunoreactivity following 24 h of culture with rosiglitazone (10 μM). Column 2 demonstrates nuclear staining (Hoechst; blue); column 3 illustrates PPAR immunoreactivity (localized using Alexa 488; green). Column 1 features overlay images. PPAR is redistributed to the nucleus in response to elevated rosiglitazone at 10 μM.

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