Cellular and Biophysical Pipeline for the Screening of Peroxisome Proliferator-Activated Receptor Beta/Delta Agonists: Avoiding False Positives

Figure 6

Quantitative TSA of PPARß/δ in different concentrations of GW0742 (a) and extract 2 (b). The curves were made using 10 μM PPARß/δ. The GW0742 concentrations were 0.3, 0.5, 1, 3, 5, 10, 30, and 50 μM, and the extract 2 concentrations were 5, 10, 30, 50, and 100 μg/mL (higher concentrations of the extract were discarded since they absorb in the used wavelengths). The temperature was varied from 20°C to 90°C in a ramp of 1°C/minute. The variations in the entire curves were approximately 16°C for GW0742 and 5°C for extract 2. Data are the mean ± SD ( independent replicates).

(a)

(b)