PPARγ Antagonizes Hypoxia-Induced Activation of Hepatic Stellate Cell through Cross Mediating PI3K/AKT and cGMP/PKG Signaling

Figure 2

PPARγ inhibits hypoxic-induced HSCs activation. (a) HSCs were exposed to hypoxia for the designated time. Protein expressions of PPARγ, α-SMA, and desmin were tested by western blotting. HSCs were exposed to hypoxia for 6 hours in the presence or absence of PPARγ agonist, rosiglitazone (RSG, 50 nM). (b) PPARγ, α-SMA, and desmin expression of HSCs in experimental groups were analyzed by western blotting. (c) α-SMA and desmin expression in HSCs in experimental groups were detected by immunofluorescence using Confocal microscopy. For each assay, . , .

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