Peroxisome Proliferator-Activated Receptor- Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5p

Figure 3

miR-590-5p transcription is regulated by nuclear translocated PPAR-γ. Preferential induction the HUVECs with or without GW9662 (5 μmol/L) for 1 h followed by the induction with Rosiglitazone (50 μmol/L) for 24 h and then determined (a) the expression of miR-590-5p, (b) the cytoplasm PPAR-γ protein level, and (c) the nucleus PPAR-γ protein level and (d) immunofluorescent staining of HUVECs using anti-PPAR-γ antibody (green) and dapi staining (purple DNA dye). (e) The expressions of miR-590-5p were determined in HUVECs induced by Rosiglitazone and Rosiglitazone-induced HUVECs transfected with PPAR-γ or plasmid lacking the nuclear localization signal (NLS) (n=3, P<0.05; vs. Control group; # P<0.05 vs. PPAR-γ with Rog treated group).

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