Peroxisome Proliferator-Activated Receptor- Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5p
Figure 4
Nuclear PPAR-γ activates the transcription of miR-590-5p through binding to its promoter. (a) A schematic diagram depicting the binding sites for PPAR-γ in the promoter of miR-590-5p. (b) ChIP assays were performed with PPAR-γ antibody and the miR-590-5p promoter region with the PPAR-γ binding motif was amplified by PCR. (c) A schematic representation of the insertion of WT-PPAR-γ or mut-PPAR-γ DNA binding motif, locating at miR-590-5p promoter region into the pMIR-REPORT Luciferase plasmid. (d) HEK-293T cells were transfected with a renilla luciferase plasmid plus a luciferase plasmid containing WT-PPAR-γ or mut-PPAR-γ promoter region; then the cells were stimulated by Rosiglitazone (PBS as negative control) for 24 h. Luciferase activities were determined by the ratio of pMIR-REPORT plasmid to pCMV-Renilla control (n=3, P<0.05).