Diet Modifies Pioglitazone’s Influence on Hepatic PPARγ-Regulated Mitochondrial Gene Expression

Figure 5

Effects of Pio on the hepatic RXR cistrome. (a) Total RXR-DNA binding sites (by group), (b) Volcano plots comparing differential RXR-DNA binding in replicate livers from Pio- versus Control- or HFD-Pio- versus HFD-treated groups of mice, and (c) Total (and unique) DNA sites with significantly different RXR-DNA binding (between groups), each defined as described in Experimental methods. (d) Venn diagram of overlap between sites of significant Pio-induced RXR-liver DNA binding in mice on control versus HFD. (e) Pie chart illustration of the locations of sites for genes summarized in (d) with respect to gene-specific transcription start sites in control versus HFD mice. (f–h) Results of corresponding RXR-liver DNA binding ChIP-Seq analyses of liver-specific PPARγ KO mice.

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